美女网站视频久久I亚洲综合区I成人手机在线视频I国产伦精品免费视频I咪咪成人网I夜夜操综合网I国产成人精品999I开心激情avI特黄一级大片I天天干天天添I在线观看网站avI88xx永久免费看大片I性欧美18一19内谢I亚洲最新视频I在线资源avI波多野结衣人妻I91porn破解版I国产又大又黄视频I欧美福利影视I四虎婷婷I性猛交xxxx乱大交孕妇印度I91丨九色丨露脸I国产又黄又骚I精品国产18久久久久久洗澡I围产精品久久久久久久I在线视频日韩一区

qiagen 74136DNA微量提取試劑盒

操作流程

RNeasy Plus Micro Kit能從多達(dá)5 x 105個(gè)細(xì)胞或5 mg組織中分離總RNA，RNeasy Plus Mini Kit能夠從107個(gè)細(xì)胞或30 mg組織中分離總RNA。簡(jiǎn)潔的工作流程可在25分鐘內(nèi)純化得到已去除基因組DNA的RNA。樣本首先被裂解和勻質(zhì)化。裂解液流過(guò)gDNA Eliminator柱，在流過(guò)gDNA Eliminator柱的溶液中加入乙醇，樣本再上樣到RNeasy離心柱。RNA結(jié)合到膜上，污染物被洗去。獲得高品質(zhì)RNA，RNeasy Plus Micro Kit的洗脫體積為14 µl，RNeasy Plus Mini Kit的洗脫體積為30 µl。

處理不同的起始樣本可用不同的實(shí)驗(yàn)方案。實(shí)驗(yàn)方案的不同之處主要在于樣本的裂解和勻質(zhì)化。樣本上樣到gDNA Eliminator柱之后的步驟就類(lèi)似。

使用Buffer RLT Plus（RNeasy Plus Micro Kit提供）進(jìn)行組織破碎和勻質(zhì)化時(shí)，可能會(huì)產(chǎn)生過(guò)多泡沫。在破碎和勻漿前向Buffer RLT Plus中加入Reagent DX（單獨(dú)提供）可充分減少泡沫產(chǎn)生。Reagent DX經(jīng)仔細(xì)檢測(cè)，表明配合此試劑盒使用不會(huì)影響RNA的純度或下游應(yīng)用。

qiagen 74136DNA微量提取試劑盒

For purification of up to 100 µg total RNA from cells/tissues using gDNA Eliminator columns

• Unique gDNA Eliminator columns avoid the need for DNase
• Efficient removal of genomic DNA
• Highly reproducible yields of RNA in minutes
• High-performance RNA for sensitive applications

The RNeasy Plus Mini Kit integrates fast, convenient purification of up to 100 µg RNA with effective elimination of genomic DNA. Cell or tissue lysates are spun through gDNA Eliminator spin columns to remove genomic DNA. Total RNA is then purified using RNeasy Mini spin columns. The kit can be automated on theQIAcube. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For smaller samples, the RNeasy Plus Micro Kit (spin-column binding capacity of 45 µg RNA) is also available.

現(xiàn)貨供應(yīng)